Newsflyer 2

aurion AURION Newsflyer 2 - part 1
DOUBLE LABEL IMMUNOHISTOCHEMISTRY for the SEPARATE OBSERVATION of TWO ANTIGENS using EPI-POLARIZATION MICROSCOPY for the IMMUNOGOLD-SILVER TECHNIQUE and FLUORESCENCE MICROSCOPY for the ALKALINE PHOSPHATASE STAINING
C.M. van der Loos

Topics

Evaluation of double staining techniques
Fluorescent and enzyme markers
Discrimination between signals and implications for photography
Fluorescent properties of alkaline phosphatase reaction products
Suitability of Gold/Silver staining in combination with alkaline phosphatase staining
Epi-polarization microscopy and fluorescent signal: two signals without overlap
Detailed description of protocols

AURION Newsflyer 2 - part 2
A COMPARISON between the STREPTAVIDIN-BIOTIN-ALKALINE PHOSPHATASE METHOD and the IMMUNOGOLD TECHNIQUE using ULTRA SMALL GOLD PARTICLES and SILVER ENHANCEMENT
R. Willemsen

Topics

Introduction to immunohistochemistry screening on blood smears
Suitability of Gold/Silver staining and alkaline phosphatase staining
Comparison of results
using alkaline phosphatase staining (3 step approach)
using immunogold silver staining (2 step and 3 step approaches)
Detailed description of protocols on blood smears

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